Absorbance Applications
- Nucleic Acid Quantitation
- Protein Quantitation
- ELISA
- Immunoassay
- Proliferation/Viability
Cartridge Details
| Cartridge | Absorbance |
| Part Number | 0200-7000 |
| Mounting | Top read (photodiode detector) and bottom read (monochromatic illumination) |
| Light Source | Flash lamp |
Paradigm Platform's advantage: flexible monochromator-based absorbance
Paradigm Platform's monochromator-based Absorbance Detection cartridge combines scanning and a broad spectrum wavelength range necessary to address absorbance applications.
Download the Absorbance Cartridge Data Sheet 
AlphaScreen Assays
AlphaScreen®, developed by Perkin Elmer, is a bead-based, proximity assay technology which requires a high-energy excitation at 680 nm and reading emission at 520-620 nm.
Typical Applications
- GPCR Assays
- Cytokine Assays
- cAMP Quantitation
- Kinase Assay
Paradigm Platform's advantage: high sensitivity, with optics to match your budget and application
Three AlphaScreen® cartridges are available for the Paradigm Platform. The Standard cartridge is ideal for occasional AlphaScreen® users. The HTS cartridge is optimized for high-sensitivity and high-throughput 96- and 384-well screens and the 1536 cartridge is optimized for the smaller optical geometry of high-density plates, avoiding cross-talk from adjacent wells.
Cartridge Details
| Cartridge | Standard | 384 HTS | 1536 HTS |
| Part Number | 0200-7017 | 0200-7018 | 0200-7019 |
| Plate Format | 96, 384 | 96, 384 | 96, 384, 1536 |
| Light Source | 680 nm laser diode | ||
| Excitation | 680 nm | ||
| Emission | 570 nm / 100 nm | ||
Download the AlphaScreen® Cartridge Data Sheet
Learning Opportunities
CatchPoint® Assays
Molecular Devices CatchPoint® Assay Kits measure cAMP or cGMP levels via a competitive immunoassay format, similar to an ELISA. The assays' high affinity reagents are optimized for sensitivity and precision in applications where cAMP or cGMP levels are low. CatchPoint assays are quantitative, allowing users to accurately determine the amount of cAMP or cGMP in samples with rapid development (less than 10 minutes) and no stop step.
Paradigm Platform's advantage: fast and simple cAMP and cGMP assays
CatchPoint® assays are fast and simple using the TUNE optical cartridge with Paradigm Platform. Using the TUNE cartridge, dedicated excitation and emission filters are not required; the appropriate wavelengths are simply selected.
Figure 1: cAMP calibration curve. The calibration curve for the CatchPoint® cAMP Assay Kit was generated on a SpectraMax® M5 Multi-Mode Microplate Reader. Data were taken 24 hours after addition of Stoplight Red substrate. The EC50 of the cAMP calibration curve was 5.1 nM.
Figure 2: Forskolin dose response in HEK293 cells. 10,000 cells were stimulated in a volume of 20 μL for 15 minutes, then lysed and analyzed using the CatchPoint® cAMP Fluorescent Assay Kit. Data were generated on a SpectraMax® M5 Multi-Mode Microplate Reader.
Cartridge Details
| Cartridge | TUNE |
| Part Number | 0200-7050 |
| Fluorophore | Stoplight Red™ |
| Excitation/Emission | Excitation: 530 nm Emission: 590 nm |
| Additional Modes | TRF, Lumi |
Download the TUNE Cartridge Data Sheet
Learning Opportunities
CatchPoint® cAMP/cGMP Assay Kits Overview
Download the CatchPoint® cAMP Reagent Data Sheet
For other luminescence applications, dedicated and multi-mode cartridges are available.
Dual Color Luminescence (BRET2) Assays
Bioluminescence Resonance Energy Transfer (BRET) is similar to FRET in that it utilizes a non-radiative energy transfer between a donor and an acceptor but rather than a fluorescent donor molecule a luminescent donor molecule is used.
Cartridge Details
| Cartridge | Dual Color Luminescence |
| Part Number | 0200-7016 |
| Emission Range | 410/80 nm, 515/30 nm |
Paradigm Platform's advantage: high-sensitivity and low cross-talk with BRET2-dedicated optics
Designed specifically for use with Perkin Elmer's BRET2 reagents, Paradigm Platform's Dual Luminescence Detection cartridge utilizes a patent-pending well-isolating design that significantly reduces the level of cross talk from surrounding wells. The resulting performance is ideal for BRET2 applications. When the cartridge is inserted, the template protocols are enabled with predefined data reduction such as blank subtraction, green to blue ratio, and Z' factor determination.
Download the BRET2 Cartridge Data Sheet
For other luminescence applications, dedicated and multi-mode cartridges are available.
Fluorescence Intensity Assays
Cartridge Details
| Cartridge | TUNE | MULTI | Fluorescein-Rhodamine | Coumarin-Fluorescein |
| Part Number | 0200-7050 | 0200-7001 | 0200-7003 | 0200-7002 |
| Typical Applications |
Any in detection range |
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| Typical Fluorophores |
Any in detection range | Coumarin Fluorescein Texas Red Rhodamine Europium (TRF only) |
Fluorescein OliGreen PicoGreen Fluo 3,4, GFP Fluorescein - Rhodamine (FRET) GFP-RFP (FRET) Alexa 488 |
Coumarin Hoescht DAPI AMCA ANS Coumarin-Fluorescein (FRET only) Alexa 350 |
| Excitation/ Emission |
Excitation: 360 nm to 790 nm Emission: 400 nm to 850 nm |
360/35 nm Ex - 465/35 nm Em 485/20 nm Ex- 535/25 nm Em 535/35 nm Ex- 595/35 nm Em 585/10 nm Ex - 635/35 nm Em 370/80 nm Ex - 616/10 nm Em |
Excitation: 485 nm/20 nm Emission: 535 nm/25 nm; 595 nm/35 nm |
Excitation: 360 nm/35 nm Emission: 465/35 nm; 535/25 nm |
| Additional Modes | TRF, Lumi | TRF, Lumi | ||
Paradigm Platform's advantage: wavelength freedom with the highest sensitivity
Paradigm Platform's TUNE cartridge supports fluorescence intensity applications at any excitation wavelength between 360 and 790 nm and any emission wavelength between 400 and 840 nm, with sensitivity up to 10x higher than other monochromator optical systems. Cartridges with specific excitation and emission filter sets are available that occupy a single bay, leaving room for additional cartridges, such as Absorbance or AlphaScreen, to be installed at the same time (three top and three bottom read positions are available).
Download the Fluorescence Intensity Cartridge Data Sheet
Download the MULTI Cartridge Data Sheet
Download the TUNE Cartridge Data Sheet
Learning Opportunities
Molecular Devices University on Microplate Assays: DNA, Protein Analysis and Immunodetection
Fluorescence Polarization Assays
Fluorescence Polarization (FP) is a homogenous assay format that relies on monitoring molecular movement and rotation. Most FP assays employ a small fluorescently-labeled molecule that binds to a larger molecule, causing a decrease in molecular rotation and a change in fluorescence polarization. FP has been widely applied to monitoring enzymatic assays.
Typical Applications
- IMAP® Kinase, Phosphatase, and Phosphodiesterase Assays
- cAMP Quantitation
- Immunoassay
- Molecular Interaction
- Nuclear Receptor
- Ligand-receptor interactions
- Interaction of Nucleic Acid Binding Proteins with Binding Sites
- Transcreener FP
- Polarscreen
- hERG
Paradigm Platform's advantage: fiber-free optics and dual PMT for high performance
FP requires monitoring light through vertical and horizontal polarizing filters in addition to fluorescence band-pass filters. Paradigm Platform's dual PMT design enables simultaneous monitoring of both vertical and horizontal emissions, delivering much faster read times compared to a single PMT design. Since polarization filters attenuate light traveling through the optical path, any optical components that further degrade light transmission can affect performance. Paradigm Platform's direct excitation optical path eliminates photon-robbing components such as optical fibers.
Cartridge Details
| Cartridge | FP-Green | FP-Red |
| Part Number | 0200-7009 | 0200-7010 |
| Typical Fluorophores | Fluorescein | Rhodamine |
| Mounting | Top or bottom read | Top or bottom read |
| Light Source | High-powered LED | High-powered LED |
| Wavelength-tuned Excitation | 485/20 nm | 535/25 nm |
| Wavelength-tuned Emission | //535/25 nm ┴535/25 nm |
//595/35 nm ┴595/35 nm |
Download the FP Cartridge Data Sheet
Learning Opportunities
Download the IMAP Technology for Kinases, Phosphatases and Phosphodiesterases Data Sheet
HTRF Assays
HTRF®, developed by CisBio, combines the features of fluorescence resonance energy transfer (FRET) and TRF, using Europium (Eu) cryptate as its donor fluorophore and a modified allophycocyanin (XL665) or the small molecule d2 as the acceptor fluorophore. When interacting biomolecules are labeled with Europium cryptate and d2, FRET takes place between the donor and the acceptor fluorophores.
Typical Applications
- GPCR Assays
- cAMP Quantitation
- Kinase Assays
- Cytokine Assays
- Human mAB Screening
Paradigm Platform's advantage: high signal-to-noise with dedicated optics, speed with dual emission detection, and ready-to-run protocols in SoftMax® Pro
HTRF® assays with Paradigm Platform are fast to run and simple to analyze. The HTRF® Detection cartridge features simultaneous emission detection, rather than sequential detection, of the donor and acceptor, yielding faster and more accurate results. Data acquisition and analysis are simple with ready-to-use protocols in the integrated SoftMax Pro software. Well-to-well variability is automatically corrected using the patented HTRF® ratio calculation (used under license). Other commonly used data reduction tools including Delta Ratio and Delta F% are also available.
Cartridge Details
| Cartridge | HTRF® |
| Part Number | 0200-7011 |
| Mounting | Top read |
| Light Source | Xenon flash lamp |
| Excitation Range | EX: HTRF |
| Emission Range | EM1 616/10 nm EM2 665/10 nm |
Download the HTRF® Cartridge Data Sheet
Learning Opportunities
WEBINAR: Secrets for Improving GPCR Binding and Functional Assessment
Molecular Devices University Training Courses on HTRF® Technology
Includes hands-on experience with the HTRF® assay platform on Molecular Devices' microplate readers.
Luminescence Assays
Paradigm Platform's advantage: choose flexible multimode cartridges or dedicated luminescence cartridges with high sensitivity and low crosstalk
Paradigm Platform has an optical cartridge to meet the needs of every glow luminescence assay, including dedicated optics for 96- through 1536-well microplates. Paradigm Platform's Luminescence Detection cartridges utilize a patent-pending well-isolating design that significantly reduces the level of cross talk from surrounding wells. The resulting performance is ideal for sensitive chemiluminescence applications. For less demanding applications, robust luminescence performance is available in cartridges that support multiple detection modes: TUNE and MULTI.
For dual-color luminescence (BRET2) assays, the BRET2 cartridge is available.
Download the Luminescence Cartridge Data Sheet
Download the MULTI Cartridge Data Sheet
Download the TUNE Cartridge Data Sheet
Cartridge Details
| Cartridge | TUNE | MULTI | LUM 96 | LUM 384 | LUM |
| Part Number | 0200-7050 | 0200-7001 | 0200-7014 | 0200-7015 | 0200-7012 |
| Typical Applications |
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| Microplate Compatibility | 96, 384 | 96, 384 | 96 | 96, 384 | 96, 384, 1536 |
| Emission Range | Visible to 800 nm | ||||
| Additional Modes | TRF, Fluorescence | N/A | |||
TRF Assays
- cAMP Quantitation
- Immunoassays
- GTP Binding
- Apoptosis
- GPCR Ligand Binding
Cartridge Details
| Cartridge | TRF |
| Part Number | 0200-7008 |
| Mounting | Top or bottom read |
| Light Source | High-powered LED |
| Wavelength-tuned excitation | 370/80 nm |
| Wavelength-tuned emission | 616/10 nm 642/10 nm |
Paradigm Platform's Advantage: High Signal to Noise with TRF-dedicated optics
Time-resolved fluorescence assays with Europium or Samarium labels are easy to optimize with Paradigm Platform's dedicated TRF optics.
Download the TRF Cartridge Data Sheet 
