Paradigm Platform
SpectraMax® Paradigm® Multi-mode Detection Platform provides the flexibility and sensitivity needed to quickly develop and run your assays, both now and in the future.
Paradigm Platform is the first reader with TUNE technology, which enables freedom to select the excitation and emission wavelengths that best fit your assay with up to 10x higher fluorescence sensitivity compared to traditional monochromator technologies. TUNE also enables spectral optimization via SoftMax Pro software with an option to automatically maximize signal-to-noise by scanning through excitation and emission wavelengths simultaneously, saving you over 50% on assay optimization time.
For superior sensitivity of specific assays, application-specific optics for HTRF, AlphaScreen and BRET2 are available. When developing an assay with novel excitation and emission wavelengths, customized filter-based cartridges are also available.
SoftMax® Pro software control and data analysis are fully integrated with Paradigm Platform. The system's unique design makes it the only user-upgradeable microplate reader on the market with the flexibility to accommodate a full range of system configurations.
Benefits
Features
General Specs
Performance Specs
- Flexible design accommodates future expansion
- User-upgradeable microplate reader; upgrade in < 2 minutes
- High speed detection
- Superior sensitivity and performance
- Automation friendly
- Future-ready
- Signal optimization with automatic Z-height optimization (top AND bottom read)
- 7 log dynamic range with patent-pending LED intensity adjustment
- Up to 1536-well format
- Temperature control
- Monochromator-based absorbance
- TUNE technology provides next-generation monochromator for FI, Lumi, TRF
General Specifications |
|
| Light Source | High-powered LED or flash lamp |
| Plate Formats | 6- to 1536-well |
| Temperature Range | 4°C above ambient to 45°C |
| Uniformity | ± 0.75°C |
| Shaking | Linear, orbital |
| Endpoint Measurement | All modes |
| Kinetic Measurement | All modes |
| Well Area Scanning | All modes |
| Wavelength Scanning | Photometry |
| Dimensions W x H x D cm (in.) | 39 (15.4) x 45 (17.7) x 63 (24.8) |
| Weight, without Cartridges | 45 kg (99 lbs) |
| Power Source | 100-240 VAC, 2 A, 50/60 Hz |
| Robotic Compatible | Automation-friendly API; StakMax compatibility |
| Linear Dynamic Range | > 5 decades |
Performance Specifications |
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Fluorescence, Luminescence, TRF Tunable Wavelength |
|
| Wavelength Scanning Range | EX 360-790 nm EM 400-850 nm |
Read Modes: Fluorescence, Luminescence, TRF |
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| Wavelength Selection | 1nm increments |
| Wavelength Accuracy | +/- 2 nm |
| Wavelength Repeatability | 1 nm |
| Bandwidth | EX/EM – 20 nm |
Fluorometric (Fluorescein) |
Optimized | Guaranteed |
| 96-well | 0.1 pM |
0.75 pM |
| 384-well | 0.15 pM | 1.3 pM |
| 1536-well | 0.4 pM | 3.7 pM |
Fluorescence Polarization (Fluorescein 1 nM, SD) |
||
| 96-well | 0.5 mP | 3 mP |
| 384-well | 0.5 mP | 3 mP |
| 1536-well | 1.5 mP | 6 mP |
Time Resolved Fluorescence (Europium) |
||
| 96-well | 0.0125 pM | 0.05 pM |
| 384-well | .01 pM | 0.065 pM |
| 1536-well | 0.08 pM | 0.19 pM |
Luminescence (ATP) |
||
| 96-well | 1.25 pM | 2.5 pM |
| 384-well | 3 pM | 6 pM |
| 1536-well | 19 pM | 25 pM |
Photometric |
|
| Wavelength Range | 230-1000 nm |
| Wavelength Selection | Monochromator (1.0 nm increments) |
| Wavelength Bandwidth | 4.0 nm |
| Wavelength Accuracy | ± 1 nm |
| Wavelength Repeatability | ± 0.5 nm |
| Photometric Range | 3.5 OD |
| Photometric Resolution | 0.0001 OD |
| Photometric Accuracy | ± (2% + 0.010 OD), 0-2 OD @ 405 nm |
| Photometric Precision | ± (1% + 0.005 OD), 0-2 OD @ 405 nm |